15:10 GMT ­ Arrived on bottom

15:26 ­ Removed wands to check electrodes 1-4.

15:35 ­ Made way to BioVent.

15:43 ­ Arrived at BioVent, positioned sub to work on Alvinella beehive x ­ 4353, y ­ 79197, heading 122.

15:52 ­ Calibrated T ­ 3.0°C from ambient.

15:54 ­ Began electrochemical/Sipper sampling of 8 different Alvinella tubes in beehive selected at BioVent. Took 125 electrochemical scans outside and within each tube, fired 8 Sippers. #1 ­ 39–47ēC, #2 ­ 65–86ēC, #3 ­ 29–92ēC, #4 ­ 77–121ēC, #5 ­ 52–73ēC, #6 ­ 25–66ēC, #7 ­ 22–40ēC, #8 ­ 22–38ēC.

16:50 ­ After sampling beehive, sampled around chimney which was collected later for ASU microcosm placement. Sipper wand electrochemistry and two Sipper samples taken here. #9 ­ ~1 inch above vent, 81ēC, #10 ­ taken at the base of the chimney, 15ēC.

17:00 ­ Began collecting of alvinellid worm samples with slurp gun at BioVent Beehive where Sipper samples 1–8 were collected. Samples were placed in ARTY chambers 1 and 2 and capped.

18:01 — RNA Later was successfully pumped into each chamber after collection finished.

18:06 ­ Use High-T probe at scar in Alvinella beehive after sampling ­ max T=58ēC at that position.

18:20 ­ Used Sipper wand to get electrochemistry and Sipper sample #11 at the scar in Alvinella beehive. Temperature at wand = 30ēC.

18:30 ­ Moved into position and collected samples for sulfide chimney samples, placed in small biobox.

18:41 ­ Used High-T probe to sample chimney, max. temperature=360ēC.

18:45 ­ Used Sipper wand to get electrochemistry and Sipper sample #12 at scar where chimney removed. Electrochem scans done at temps up to 207ēC (bear in mind thermistor located ~1cm in front of electrode).

18:52 ­ Used macro wand in plume above chimney scar, positioned in three points across range — 25, 58, 62ēC.

19:00 ­ Positioned ASU unit on top of scar.

19:05 ­ Used macro wand above ASU chimney where sulfide plume coming out.

19:13 ­ Finished work at BioVent Alvinella beehive, moved 15 m to collect clams.

19:16 ­ Finished positioning for clam collection x ­ 4359, y ­ 79188.

19:46 ­ Power low after collecting eight clams, moved away from site to drop weights and come up.