Extreme 2004: Exploring the Deep Frontier Search

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Frank Stewart

Extreme Crew

Where are you from, and what is your role in Extreme 2004?

I am a second-year graduate student working with Dr. Colleen Cavanaugh at Harvard University. Our lab will be using molecular techniques to study microorganisms found in association with hydrothermal vents and in symbiotic relationships with vent invertebrates.

What kinds of questions will you try to answer, and why?

We are intersted in the bacterium that grows in a symbiotic relationship with the vent tubeworm Riftia pachyptila. This yet-to-be-cultured bacterium can
synthesize its own food (organic carbon) using the chemicals in vent fluids as an energy source. In so doing, the bacterium then provides a vital food resource for the host tubeworm. On this cruise, we will try to identify where this important bacterium is found outside of its tubeworm host. To do so, we will collect water and invertebrate (tubeworms, clams, mussels) samples as well as deploy and retrieve devices designed to collect bacteria that settle from the water column. Many of our samples will be preserved aboard ship and then shipped home for genetic analysis. In characterizing the distribution of the "free-living" Riftia symbiont, we will provide information about how the tubeworm acquires its symbiont and about how this important symbiosis may have evolved.

What is your educational background, what lured you into marine research?

As a kid, I became interested in marine life by keeping saltwater aquariums and by exploring the rich tide pools on the Oregon coast. In 2000, I received a B.A. in biology from Middlebury College. I then went on to study bacteria in Antarctic sea ice as a graduate student at the University of Nevada, Reno, where I received an M.S. in environmental science in 2002. This will be my seventh research cruise ... and my second hydrothermal vent cruise.

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