People | Kathryn J. Coyne

• Ph.D., Biochemistry, University of Delaware, 1997.
• B.S., Chemistry, University of Delaware, 1986.

Biochemical and molecular ecology of harmful algal bloom species; development of biological indicators; production of algal biofuel coupled to bioremediation

For details about current projects, please visit our lab website:

Microbial Ecology Research Laboratory (MERL) Website

www.udel.edu/MERL/index.html

• Biofuel Production Coupled to Bioremediation of Industrial Flue Gas
• The Vibrio-HAB Connection
• Detection Methods for Enumeration of HABs
• Algicidal Agent Produced by Shewanella sp.
• Transcriptome analysis of raphidophytes, Chattonella subsalsa and Heterosigma akashiwo

Jennifer Stewart, jen@udel.edu, USDA postdoctoral fellow. Jen is characterizing expression patterns and enzyme activities of a novel nitrate reductase from the raphidopohyte alga, Heterosigma akashiwo.

Colleen Bianco, cbianco@udel.edu, Master's student, UD. Colleen is evaluating changes in lipid, carbohydrate and protein content of algae grown on flue gas mixtures.

Kaytee Pokrzywinski, kaytee@udel.edu, Ph.D. student, UD.  Kaytee is investigating the effects of an algicidal compound on dinoflagellate growth and physiology.

Christopher Main, cmain@udel.edu, Ph.D. student, UD.  Chris is developing molecular methods to evaluate interactions between pathogenic Vibrio species and harmful algal blooms.

Demir-Hilton, E., Hutchins, D. A., Czymmek, K. J. and Coyne, K. J. (2012) Description of Viridilobus marinus (gen. et sp. nov.), a new raphidophyte from Delaware's inland bays. Journal of Phycology 48:1220-1231.

Pokrzywinski, K. L., Place, A. R., Warner, M. E. and Coyne, K. J. Investigation of the algicidal exudate produced by Shewanella sp. IRI-160 and its effect on dinoflagellates. Harmful Algae 19:23-29.

Stewart, J. J. and Coyne, K. J. (2011) Analysis of raphidophyte assimilatory nitrate reductase reveals unique domain architecture incorporating a 2/2 hemoglobin. Plant Molecular Biology 77:565-575.

Gobler, C. J., Berry, D. L., Dyhrman, S. T., Wilhelm, S. W., Salamov, A., Lobanov, A. V., Zhang, Y., Collier, J. L., Wurch, L. L., Kustka, A. B., Dill, B. D., Shah, M., VerBerkmoes, N. C., Kuo, A., Terry, A., Pangilinan, J., Lindquist, E. A., Lucas, S., Paulsen, I. T., Hatternrath-Lehmann, T. K., Talmage, S. C., Walker, E. A., Koch, F., Burson, A. M., Marcoval, M. A., Tang, Y.-Z., LeCleir, G. R., Coyne, K. J., Berg, G. M., Bertrand, E. M., Saito, M. A., Gladyshev, V. N., Grigoriev, V. N. (2011) Niche strategy of harmful algae revealed through comparative genomics of competing phytoplankton species. Proceedings of the National Academy of Sciences 108:4352-4357.

Fredrickson, K.A., Strom, S. L., Crim, R. and Coyne, K. J. (2011) Inter-strain variability in physiology and genetics of Heterosigma akashiwo (Rahpidophyceae) from the west coast of North America. Journal of Phycology 47:25-35.

Coyne, K. J. (2010) Nitrate reductase (NR1) sequence and expression in the harmful alga Heterosigma akashiwo (Raphidophyceae). Journal of Phycology 46:135-142.

Portune, K. J., Coyne, K. J., Hutchins, D. A., Handy, S. M. and Cary, S. C. (2009) Germination of Heterosigma akashiwo and Chattonella subsalsa (Raphidophyceae) cysts in Delaware’s Inland Bays, DE.  Aquatic Microbial Ecology 55:229-239.

Demir, E., Coyne, K. J., Doblin, M. A., Handy, S. M. and Hutchins, D. A.  (2008) Microzooplankton grazing rates on Heterosigma akashiwo in the Delaware Inland Bays: A species-specific approach combining quantitative real-time PCR (QPCR) and dilution methods.  Microbial Ecol. 55: 583-594.

Handy, S. M., Demir, E., Hutchins, D. A., Portune, K. J., Whereat, E. B., Hare, C. E., Rose, J. M., Warner, M., Farestad, M., Cary, S. C., and Coyne, K. J. (2008) Using quantitative real-time PCR to study competition and community dynamics among Delaware Inland Bays harmful algae in field and laboratory studies. Harmful Algae 7:599-613.

Doblin, M. A., Coyne, K. J., Rinta-Kanto, J. M., Wilhelm, S. W., and Dobbs, F. C. (2007) Dynamics and short-term survival of cyanobacteria species in ballast water—implications for HAB invasions. Harmful Algae 6:519-530.

Gobler, C. J., Davis, T. W., Coyne, K. J., and Boyer, G. L. (2007) The interactive influences of nutrient loading and zooplankton grazing on the growth and toxicity of cyanobacteria blooms in a eutrophic lake.  Harmful Algae 6:119-133.

Coyne, K. J., Hare, C. E., Popels, L. C., Hutchins, D. A., and Cary, S. C. (2006) Distribution of Pfiesteria piscicida cyst populations in sediments of the Delaware Inland Bays, USA. Harmful Algae 5:363-373.

Handy, S. M., Cary, S. C., Hutchins, D. A., and Coyne, K. J. (2006) Simultaneous enumeration of multiple raphidophyte species by quantitative real-time PCR: capabilities and limitations. Limnol. Oceanog. Methods 3:193-204.

Zhang, Y., F.-X. Fu, E., Whereat, K.J. Coyne and D. A. Hutchins. (2006) Bottom-up controls on a mixed-species HAB assemblage:  A comparison of sympatric Chattonella subsalsa and Heterosigma akashiwo (Raphidophyceae) isolates from the Delaware Inland Bays, USA.  Harmful Algae 5:310-320.